Makler Counting Chamber (With Non Gridded Glass)

The Makler Counting Chamber stands out with its exceptional depth of just 10 microns—one-tenth that of standard hemocytometers—making it the shallowest chamber available. This design is achieved through its construction from two pieces of optically flat glass. Specifically, the Makler Counting Chamber features an upper cover glass with a 1 sq. mm grid, subdivided into 100 squares of 0.1 x 0.1 mm each. Additionally, the spacing is firmly maintained by four quartz pins.

Analysis Technique: To begin, place a small, uncalibrated drop from a well-mixed specimen into the center of the Makler Counting Chamber using a simple rod. Following this, cover the chamber and use a x20 microscopic objective for detailed analysis.

Motility Evaluation: Next, count non-motile sperm within nine or sixteen squares in the grid’s center. Subsequently, track moving sperm and, if needed, grade them. Repeat this process in several areas to ensure comprehensive evaluation. This allows for accurate calculation of both the percentage and quality of motility.

Advantages:

• Firstly, spermatozoa are uniformly distributed and monolayered, allowing observation in a single focal plane.
• Moreover, dilution is unnecessary, even with concentrated specimens; analysis is conducted directly from the original specimen.
• Additionally, all spermatozoa acquire friction-free horizontal movement, examined under consistent conditions.
• Consequently, the specimen can be analyzed quickly, making it feasible as an office procedure while the patient waits, even for those with limited experience.
• Furthermore, the accuracy of analysis is enhanced by eliminating various steps required by traditional hemocytometric techniques. Ensuring that sperm motility is assessed under identical conditions further improves accuracy, preventing errors caused by uncontrolled cover slip pressure.
• Importantly, the 10-micron depth of the Makler Counting Chamber is ideal for photomicrography, as it aligns with the field depth of the objective used in semen analysis.
• Finally, the chamber’s design allows for quick and efficient reuse, making it suitable for busy laboratories. A single technician can perform numerous tests per hour with minimal technical and material requirements.